A.Mallamaci, 22 hours

• Goals of manipulations, gateways to the genome, molecular machineries

• Germline transgenesis in zygote: classical and lentiviral

• Gene trapping: NHEJ- and LV- assisted in ESCs, PiggyBac trapping in zygotes

• Site-specific recombinases and their use in conditional mutagenesis

• Conditional transgenesis by ligand-regulatable transcription

• Gene editing, classical in ESCs

• Engineered endonucleases (ZFNs, TALENs, CRISPR) and their use in gene editing

• DNA base editing

• Modulation of endogenous gene expression by programmable epigenetic effectors, programmable transcription factors, small activating RNAs and RNAi effectors

• Modulation of endogenous gene expression by programmable translational transactivators (SineUps) and polypeptide Small Molecule-Assisted Shutoff (SMASh)