A.Mallamaci, 22 hours
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Goals of manipulations, gateways to the genome, molecular machineries
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Germline transgenesis in zygote: classical and lentiviral
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Gene trapping: NHEJ- and LV- assisted in ESCs, PiggyBac trapping in zygotes
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Site-specific recombinases and their use in conditional mutagenesis
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Conditional transgenesis by ligand-regulatable transcription
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Gene editing, classical in ESCs
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Engineered endonucleases (ZFNs, TALENs, CRISPR) and their use in gene editing
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DNA base editing
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Modulation of endogenous gene expression by programmable epigenetic effectors, programmable transcription factors, small activating RNAs and RNAi effectors
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Modulation of endogenous gene expression by programmable translational transactivators (SineUps) and polypeptide Small Molecule-Assisted Shutoff (SMASh)